DNA AND BSA INTERACTIONS OF COPPER(II) AND ZINC(II) COMPLEXES WITH ANTIFUNGAL AGENT FLUCONAZOLE

1st International Conference on Chemo and BioInformatics, ICCBIKG  2021, (399-402)

AUTHOR(S) / АУТОР(И): Nevena Lj. Stevanović, Mia Stanković, Tina P. Andrejević, Darko P. Ašanin, Ivana M. Stanojević, Biljana Đ. Glišić

E-ADRESS / Е-АДРЕСА: nevena.stevanovic@pmf.kg.ac.rs,tina.andrejevic@pmf.kg.ac.rs, biljana.glisic@pmf.kg.ac.rs, mia.v@ucg.ac.me, darko.asanin@uni.kg.ac.rs, stanojevic.ivana@ni.ac.rs

Download Full Pdf   

DOI: 10.46793/ICCBI21.399S

ABSTRACT / САЖЕТАК:

Aromatic nitrogen-containing heterocycles (N-heterocycles) have attracted a considerable attention as scaffolds for compounds, which have an application in different pharmacological areas, ranging from vitamins to different antimicrobial and antitumor agents. In this respect, azoles are of special importance as potent and broad-spectrum agents used for the treatment of many invasive fungal infections. In the present study, the interaction of the clinically used antifungal drug fluconazole (fcz) and its copper(II) and zinc(II) complexes, {[CuCl2(fcz)2].5H2O}n (1) and {[ZnCl2(fcz)2]·2C2H5OH}n (2), with calf thymus DNA (ct- DNA) and bovine serum albumin (BSA) has been investigated. Fluorescence emission spectroscopy was applied for the binding study of complexes 1 and 2 and fcz with ct-DNA and BSA, while cyclic voltammetry was additionally used for investigation of their interactions with ct-DNA. The values of calculated binding constants (KA) of the investigated compounds towards ct-DNA and BSA follow the order fcz < 1 < 2 and 2 < fcz < 1, respectively.

KEY WORDS / КЉУЧНЕ РЕЧИ:

copper(II) complex, zinc(II) complex, fluconazole, ct-DNA interaction, BSA interaction

REFERENCES / ЛИТЕРАТУРА:

  • G. Gasser, N. Metzler-Nolte., The Potential of Organometallic Complexes in Medicinal Chemistry, Current Opinion in Chemical Biology, 16 (2012) 84-91.
  • J. A. Maertens., History of the Development of Azole Derivatives, Clinical Microbiology, and Infection, 10 (2004) 1-10.
  • N. Lj. Stevanović, I. Aleksic, J. Kljun, S. Skaro Bogojevic, A. Veselinovic, J. Nikodinovic- Runic, I. Turel, M.I. Djuran, B.Đ. Glišić, Copper(II) and Zinc(II) Complexes with the Clinically used Fluconazole: Comparison of Antifungal Activity and Therapeutic Potential, Pharmaceuticals, 14 (2021) 24.
  • J-H. Shi, D.-Q. Pan, M. Jiang, T.-T. Liu, Q. Wang., In Vitro Study on Binding Interaction of Quinapril with Bovine Serum Albumin (BSA) using Multi-Spectroscopic and Molecular Docking Methods, Journal of Biomolecular Structure and Dynamics, 35 (2017) 2211-2223
  • V. Anbazhagan, R. Renganathan., Study on the Binding of 2,3-Diazabicyclo [2.2.2]oct-2- ene with Bovine Serum Albumin By Fluorescence Spectroscopy, Journal of Luminescence, 128 (2008) 1454-1458.
  • , Interaction between DNA and Microcystin-LR Studied by Spectra Analysis and Atomic Force Microscopy, Biomacromolecules, 12 (2011) 797-803.