1st International Conference on Chemo and BioInformatics, ICCBIKG  2021, (343-346)

AUTHOR(S) / АУТОР(И): Marina M. Stanković, Jelena Z. Pribojac, Jelena N. Terzić, Olgica D. Stefanović

E-ADRESS / Е-АДРЕСА: marina.stankovic.93@gmail.com, jelena91p@gmail.com, jelenapn93@hotmail.com, olgica.stefanovic@pmf.kg.ac.rs

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DOI: 10.46793/ICCBI21.343S


Mentha piperita and Melissa officinalis are both well-known medicinal plants that have applications in traditional medicine. In this research the antibacterial activity of the ethanol extracts of M. piperita and M. officinalis was examined against 14 bacterial strains via the microdilution method. Minimum inhibitory concentrations of ethanol extracts of both plant species ranged from 0.312 to 20 mg/mL. Standard strains of Staphylococcus aureus ATCC 25923 at a concentration of 0.312 mg/mL and Bacillus subtilis ATCC 6633 at a concentration of 1.25 mg/mL showed the highest sensitivity to the ethanol extract of M. piperita. Ethanol extract of M. officinalis showed antibacterial activity on standard strains of S. aureus ATCC 25923 and B. subtilis ATCC 6633 at a concentration of 0.625 mg/mL. In addition to the mentioned standard strains, it showed activity on the isolate from the food Proteus spp. at a concentration of 0.312 mg/mL and isolate from the wound Proteus mirabilis at a concentration of 0.625 mg/mL. Mechanism of action of the ethanol extract of M. officinalis was examined on the permeability of the bacterial cell membrane. The effect of the extract on the increased permeability of the cell membrane was measured based on the release of proteins and the percentage of crystal violet binding. Ethanol extract of M. officinalis has been shown to act at the level of the cell membrane in the following bacterial strains of Pseudomonas aeruginosa, S. aureus and Enterococcus spp.


Mentha piperita, Melissa officinalis, antibacterial activity, mechanism of action, permeability of the cell membrane


  • A. Madeiro, N.H. Borges, A.L. Souto, P.T. de Figueiredo, J.P. Siqueira-Junior, J.F. Tavares., Modulation of the antibiotic activity against multidrug resistant strains of coumarins isolated from Rutaceae species, Microbial Pathogenesis, 104 (2017) 151-154.
  • S. Nagi., Plant extracts for the control of bacterial growth: Efficacy, stability and safety issues for food application, International Journal of Food Microbiology, 156 (2012) 7-17.
  • C. Wootton-Beard, A. Moran, L. Ryan., Stability of the total antioxidant capacity and total polyphenol content of 23 commercially available vegetable juices before and after in vitro digestion measured by FRAP, DPPH, ABTS and FolineCiocalteu methods, Food Research International, 44 (2011) 217-224.
  • Quettier-Deleu, B. Gressier, J. Vasseur, T. Dine, C. Brunet, M. Luyckx, M. Cazin, J.C. Cazin, Bailleul, F. Trotin., Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour, Journal of Ethnopharmacology, 72 (2000) 35-42.
  • P.S. Makkar, M. Blummel, N.K. Borowy, K. Becker., Gravimetric determination of tannins and their correlations with chemical and protein precipitation method, Journal of Science of Food and Agriculture, 61 (1993) 161–165.
  • J. Porter, L.N. Hrstich, B.G. Chan., The conversion of procyanidins and prodelphinidins to cyanidin and delphinidin, Phytochemistry, 25 (1986) 223-230.
  • D. Sarker, L. Nahar, Y. Kumarasamy., Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals, Methods, 42 (2007) 321-324.Vaara, T. Vaara., Outer Membrane Permeability Barrier Disruption by Polymyxin in Polymyxin-Susceptible and – Resistant Salmonella typhimurium, Antimicrobial Agents and Chemoterapy, 19 (1981) 578-583.
  • Stefanović, Lj. Čomić., Synergistic antibacterial interaction between Melissa officinalis extracts and antibiotics, Journal of Applied Pharmaceutical Science, 02 (2012) 01-5.
  • Atanassova, S. Georgieva, K. Ivancheva., Total phenolic and total flavonoid contents, antioxidant capacity and biological contaminants in medicinal herbs, Jouranal of the University of Chemical Technology and Metallurgy, 46 (2011) 81-88.
  • Ö. Ertürk., Antibacterial and antifungal activity of ethanolic extracts from eleven spice plants, Biologia, 61 (2006) 275-278.
  • Bupesh, S. Amutha, S. Nandagopal, A. Ganeshkumar, P. Sureshkumar, K. Saravana Murali., Antibacterial activity of Mentha piperita L. (peppermint) from leaf extracts – a medicinal plant, Acta Agriculturae Slovenica, 89 (2007) 73-79