SYNTHESIS AND BIOLOGICAL ACTIVITY OF THE NEW PINCER TYPE RUTHENIUM(III) COMPLEX

1st International Conference on Chemo and BioInformatics, ICCBIKG  2021, (316-319)

AUTHOR(S) / АУТОР(И): Ana Rilak Simović, Dejan Lazić, Milica Međedović, Dušan Ćoćić, Biljana Petrović

E-ADRESS / Е-АДРЕСА: anarilak@kg.ac.rs, dlazic.kg@gmail.com, milica.medjedovic@pmf.kg.ac.rs, dusan.cocic@pmf.kg.ac.rs, biljana.petrovic@pmf.kg.ac.rs

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DOI: 10.46793/ICCBI21.316RS

ABSTRACT / САЖЕТАК:

We synthesized and characterized the ruthenium(III) pincer-type complex [RuCl3(H2Lt-Bu] (H2Lt- Bu = 2,6-bis(5-tert-butyl-1H-pyrazol-3-yl)pyridine, 1) by elemental analysis, IR and UV-Vis spectroscopy, and mass spectrometry (MS) method ESI Q-TOF. For comparison reason, we also studied ruthenium(III) terpyridine complexes of the general formula [Ru(N-N-N)Cl3] where N-N-N = 4′-chloro- terpyridine (Cl-tpy; 2) or 4′-chlorophenyl-terpyridine (Cl-Ph-tpy; 3). Kinetic study of the substitution reactions of 1–3 with biomolecules showed that the rate constants depend on the properties of the spectator ligand and the nature of the entering nucleophile. To gain further insight into the reactivity of ruthenium complexes with potential biological targets, we examined the interactions of 1 – 3 with DNA and HSA. The DNA/HSA binding study showed that in comparison to complex 1 (bis– pyrazolylpyridine), the other two (2 and 3) terpyridine complexes had a slightly better binding affinity to calf thymus DNA (CT DNA), while in the case of human serum albumin (HSA), complex 1 exhibited the most strong quenching ability.

KEY WORDS / КЉУЧНЕ РЕЧИ:

ruthenium(III), site markers, DNA, HSA

REFERENCES / ЛИТЕРАТУРА:

  • Rilak Simović, R. Masnikosa, I. Bratsos, E. Alessio, Chemistry and reactivity of ruthenium(II) complexes: DNA/protein binding mode and anticancer activity are related to the complex structure, Coordination Chemistry Reviews, 398 (2019) 113011.
  • Brabec, J. Kasparkova, Ruthenium coordination compounds of biological and biomedical significance. DNA binding agents, Coordination Chemistry Reviews, 376 (2018) 75-94.
  • Masnikosa, M.M. Milutinović, I. Crnolatac, A. Tot, S. Veličković, Ž. Bojić-Trbojević, A. Rilak Simović, Anti-adhesive action of novel ruthenium(II) chlorophenyl terpyridine complexes with a high affinity for  double-stranded DNA: in vitro and in silico, Journal of Inorganic Biochemistry, 208 (2020) 111090.