1st International Conference on Chemo and BioInformatics, ICCBIKG 2021, (259-262)
AUTHOR(S) / АУТОР(И): Nevena Milivojević, David Caballero, Mariana R Carvalho, Marko Živanović, Nenad Filipović, Rui L Reis, Joaquim M Oliveira
E-ADRESS / Е-АДРЕСА: email@example.com, firstname.lastname@example.org, email@example.com, firstname.lastname@example.org, email@example.com, firstname.lastname@example.org,
ABSTRACT / САЖЕТАК:
Further technological advances are in great need for improving our understanding about critical biological and fundamental pathological processes, such as tissue development and cancer progression, or for the discovery and screening of novel pharmacological drugs. Preclinical experimentation demands for highly reliable and physiologically-relevant systems capable of recapitulating the complex human physiology. Traditional in vitro models, albeit widely employed, fail to reproduce the complexity of the native scenario with cells displaying aberrant gene expressions. Similarly, in vivo animal models, such as mice, poorly mimic the human condition and are ethically questionable. During the last decades, a new paradigm in preclinical modelling has emerged aiming to solve the limitations of the aforementioned methods. The combination of advanced tissue engineering, nanotechnology, and cell biology has resulted in the development of cutting-edge microfluidics-based models with an unprecedented ability to recreate within a microfluidic device the native habitat of cells within a microengineered chip. A diverse variety of micro- and bio-fabrication techniques is available for the development of microfluidic devices. Among all them, UV-photolithography and soft lithography is the considered the gold-standard method for the fabrication of chips due to its simplicity, versatility, and rapid prototyping. In this work, we describe the step-by-step fabrication procedure of a microfluidic chip by UV-photolithography and replica molding and discuss about their potential applications in the biomedical field.
KEY WORDS / КЉУЧНЕ РЕЧИ:
microfluidics, lab-on-chip, microfabrication, UV-photolithography, replica molding
REFERENCES / ЛИТЕРАТУРА:
- Carvalho MR, Barata D, Teixeira LM, Giselbrecht S, Reis RL, Oliveira JM, Truckenmüller R, Habibovic P. Colorectal tumor-on-a-chip system: A 3D tool for precision onco-nanomedicine.Sci Adv. 2019 May 22;5(5): eaaw1317. doi: 10.1126/sciadv.
- Zhang YS, et al. Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors. Proc Natl Acad Sci U S A. 2017 Mar 21;114(12): E2293- E2302. doi: 10.1073/pnas.1612906114.
- David Caballero, Maria AngélicaLuque-González, Rui L. Reis, Subhas C. Kundu, Chapter 15 – Microfluidic systems in cancer research, Biomaterials for 3D Tumor Modeling, Elsevier, 2020, Pages 331-377, ISBN 9780128181287, doi: 1016/B978-0-12-818128-7.00015-0.
- Bower R, Green VL, Kuvshinova E, Kuvshinov D, Karsai L, Crank ST, Stafford ND, Greenman Maintenance of head and neck tumor on-chip: gateway to personalized treatment? Future Sci OA. 2017 Mar 7;3(2): FSO174. doi: 10.4155/fsoa-2016-0089.
- Carvalho, R., Carvalho, C.R., Maia, F.R., Caballero, D., Kundu, S.C., Reis, R.L. and Oliveira, J.M. (2019), Peptide-Modified Dendrimer Nanoparticles for Targeted Therapy of Colorectal Cancer. Adv. Therap., 2: 1900132. doi: 10.1002/adtp.201900132.
- Gumuscu B, Albers HJ, van den Berg A, Eijkel JCT, van der Meer AD. Compartmentalized 3D Tissue Culture Arrays under Controlled Microfluidic Delivery. Sci Rep. 2017 Jun 13;7(1):3381. doi: 1038/s41598-017-01944-5.